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2 edition of Electrophysiology of maturing oocytes from the frog, Rana pipiens. found in the catalog.

Electrophysiology of maturing oocytes from the frog, Rana pipiens.

Lyanne Clemens Schlichter

Electrophysiology of maturing oocytes from the frog, Rana pipiens.

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  • 12 Currently reading

Published .
Written in English


The Physical Object
Pagination317 leaves.
Number of Pages317
ID Numbers
Open LibraryOL14721087M

  Free Online Library: Histology reveals testicular oocytes and trematode cysts in the threatened Oregon spotted frog (Rana pretiosa).(Report) by "Northwestern Naturalist: A Journal of Vertebrate Biology"; Biological sciences Flukes Health aspects Frogs Oocytes. Maturation-promoting factor (abbreviated MPF, also called mitosis-promoting factor or M-Phase-promoting factor) is the cyclin-Cdk complex that was discovered first in frog eggs. It stimulates the mitotic and meiotic phases of the cell cycle. MPF promotes the entrance into mitosis (the M phase) from the G 2 phase by phosphorylating multiple proteins needed during mitosis. MPF is activated at. “The Experimental Production and Development of Triploid Frog Embryos.” Journal of Experimental Zoology (): – “The Influence of Egg Volume on the Development of Haploid and Diploid Embryos of the Frog, Rana pipiens.”Journal of Experimental Zoology (): – With Rufus R. Humphrey and G. Fankhauser.


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Electrophysiology of maturing oocytes from the frog, Rana pipiens. by Lyanne Clemens Schlichter Download PDF EPUB FB2

Anatomy and Physiology of Vision in the Frog (Rana pipiens) Humberto R. Maturana, J. Lettvin, W. McCulloch, and W. Pitts From the Research Laboratory of Electronics, Massachusetts Institute of Technology, CambridgeCited by: “The frog” is, in this case, the northern leopard frog, Rana pipiens.

To facilitate description of the electrical properties of the oocyte and to make this chapter of broader usefulness I begin with a description of the resting membrane (voltage and resistance) and give many technical tips on making and interpreting such measurements Cited by: 5.

Appendix: Chronological Summary Rana pipiens. book Organ Anlagen Appearance of Rana pipiens Stage 3 mm total body length. Blastopore closes, neurenteric canal transient, neural tube closed but no differentiation of central nervous system, body elongated with back curve changing from convex to concave, myotomes evident but movement by surface ciliation.

Spermatogenesis in the frog: Rana pipiens. (A) Testis of a recently metamorphosed frog. (B) Similar to "A" except that the frog was previously treated with the anterior pituitary hormone to evacuate the seminiferous tubules, leaving only the interstitial tissue, spermatogonia, and a few primary spermatocytes.

This chapter represents a personal view of the frog oocyte during maturation and fertilization, as seen through the eyes of an electrophysiologist. “The frog” is, in this case, the northern leopard frog, Rana pipiens.

MATURING oocytes of the frog Rana pipiens arrest in prophase of the first meiotic division, when they can be isolated and induced to complete meiotic maturation in vitro by exposure to steroid Cited by: Cleavage'' and cortical granule breakdown in Rana pipiens oocytes induced by direct microinjection of calcium.

DEVELOPMENTAL BIOL () Conservation of RNA Polymerase during Maturation of the Rana pipiens Oocyte T. HOLLINGEE1 AND L. SMITH2 The University of Tennessee-Oak Ridge Graduate School of Biomical Sciences and Biology Division, Oak Ridge National Laboratory,3 Oak Ridge, Tennessee ; and the Department of Biological Sciences, Purdue University, W.

Cited by: Oocytes of the leopard frog, Rana pipiens, contain soluble tubulin which was previously shown to exist predominantly in megadalton (MDa) fractions and that fails to readily assemble in vitro.

When two frog species, Rana pipiens and Rana sylvatica, mate, the offspring die early in embryonic development. This is an example of _____.

mechanical isolation behavioral isolation temporal isolation hybrid inviability hybrid sterility. Assisted Reproductive Technology. Stem cells. Molecular. The northern leopard frog (Lithobates pipiens or Rana pipiens) is a species of leopard frog from the true frog family, native to parts of Canada and the United States.

It is the state amphibian of Minnesota and Vermont. 2 Color variations. 3 Ecology and behavior. Neuroscience. Muscle physiology and biomechanics. 9 Further : Ranidae. EXPERIMENTAL CELL RESEARCH() Effects of Acrylamide on Germinal Vesicle Migration and Dissolution in Oocytes of the Frog, Rana pipiens CHARLES A.

LESSMAN*'1 AND RICHARD G. KESSELf ^'Department of Biology, Memphis State University, Memphis, Tennessee ; and ^Department of Biology, University of Iowa, Iowa City, Iowa We have demonstrated that -when Rana oocytes Cited by: 8. Manual dissec- tion of oocytes from their follicles does not, however, remove all adherent follicle cells (Smith et al., ).

Complete removal of the adherent follicle cells by chemical methods prevents maturation induced by OOCYTE NUCLEUS IN R. pipiens MATURATION pituitary homogenates in vitro (Masui, ).Cited by: DNA-dependent RNA polymerase was extracted from oocytes of the frog, Rana pipiens.

The bulk of the enzyme activity was present in the germinal vesicle and the amounts of each major form of such activity did not significantly change during oocyte maturation.

Therefore, either nuclear polymerase. DIFFERENTIATION OF THE CORTICAL CYTOPLASM AND INCLUSIONS IN OOCYTES OF THE FROG Norman E. Kemp. Norman E. Kemp From the Department of Zoology, University of Michigan, Ann Arbor CORTICAL CHANGES IN GROWING OOCYTES AND IN FERTILIZED OR PRICKED EGGS OF RANA by: Abstract.

The susceptibility of the frog embryo to infective transmission of the renal adenocarcinoma was shown following inoculation of R. pipiens embryos with subcellular fractions and filtrates from inclusion body tumors (1).

The cytoplasmic fractions injected were rich Cited by: 7. The Frog Its Reproduction and Development. By Roberts Rugh, Ph.D. The Blakiston Company General Introduction to the Embryology of the Leopard Frog, Rana pipiens; Chapter Three - Reproductive System of the Adult Frog: Rana pipiens The seed for this book was planted early in the author's mind while he was being initiated into the.

The folded cortex of the growing oocyte of the frog extends as microvilli into the substance of the developing vitelline membrane and, internal to the folds, possesses a layer of cortical granules.

Free ribosomes, smooth-walled vesicles, coated vesicles, tubules, and electron-opaque granules are abundant in the peripheral zone of the by:   Immature oocytes of the Rana perezi frog were studied electrophysiologically to see if some of the unusual ionic channels found in Xenopus oocytes were also expressed in these cells.

Growing oocytes showed a fairly linear current/voltage relationship (from – to +60 mV), whereas fully grown cells had several voltage-dependent by: Abstract.

Seven nuclear lines derived from erythrocyte nuclei of Rana pipiens were produced by serial nuclear transplantation into oocytes and eggs. Even at the termination of the experiments, embryos and tadpoles developed in the eighth transplant generations. Thus, there was no evidence that the mitotic progeny of the erythrocyte nuclei lost their Cited by: Oocytes of Rana pipiens exposed to exogenous progesterone in order to induce maturation have been observed to extensively metabolize this hormone.

When progesterone was injected directly into the oocytes, they did not mature, but similar metabolism of progesterone occurred. The metabolites have been tentatively identified as the 5α-reduced derivatives, 5α-pregnanedione, 5α-pregnanα-ol-3 Cited by: Request PDF | Soluble tubulin complexes in oocytes of the common leopard frog,Rana pipiens, contain?-tubulin | Oocytes of the leopard frog, Rana pipiens, contain soluble tubulin which was.

Start studying Bio Final Mastering Biology CH Learn vocabulary, terms, and more with flashcards, games, and other study tools. When two frog species, Rana pipiens and Rana sylvatica, mate, the offspring die early in embryonic development.

Reduced hybrid viability. Three species of frogs—Rana pipiens, Rana clamitans, and Rana. The following experiments were, therefore, undertaken in an attempt to elucidate the roles of cytoplasmic molecules as putative regulatory factors of chromosome behavior in the frog oocyte.

METHODOLOGY Animals. Mature Rana pipiens kept in 10% Ringer solution under hibernating conditions (2–4°C) were used for experiments. Oocytes and eggsCited by:   Microinjection of approximately mug of calcium into maturing oocytes of Rana pipiens after nuclear dissolution resulted in cleavage- like constrictions, cortical granule breakdown, and formation of a structure resembling a two-cell embryo.

Mg2+, Na+, or K+ did not induce any of. Fertilization of the frog egg (Rana pipiens) elicits a positive-going shift in membrane potential (fertilization potential) that lasts min and functions as a fast block to polyspermy. We examined the ion conductances underlying the fertilization potential, using the voltage-clamp by: 29) Wang, T.

and Lessman, C.A. () Tubulin dynamics during oocyte maturation in the frog, Rana pipiens. Developmental Biology 28) Woods, C.W. and Lessman, C.A.

() Antibody microinjection into maturing oocytes of the amphibian genus Rana. Journal of the Tennessee Academy of Science a. cortical changes in growing oocytes and in fertilized or pricked eggs of rana pipiens Norman E.

Kemp and Nancy L. Istock From the Department of Zoology, The University of Michigan, Ann Arbor Cited by: Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells Article (PDF Available) in Nature () October with Reads How we measure 'reads'.

However, recent books show them as eastern and western forms of a single species, the yellow-rumped warbler. When two frog species, Rana pipiens and Rana sylvatica, mate, the offspring die early in embryonic development.

Three species of frogs—Rana pipiens, Rana clamitans, and Rana sylvatica— all mate in the same ponds, but they. Oocyte Rana pipiens Ultrastructure Cytochemistry Enzymes This investigation was supported by a research grant from the National Science Foundation (GB). This is a preview of subscription content, log in to check by: 8.

The oocyte of Rana pipiens is coated by three visible jelly layers designated V1, V2, and V3 from inner to outer. Oocytes with V1 were rarely fertilized, while oocytes with V1 and V2, V1 and V3. Amphinase is a type of a ribonuclease enzyme isolated from the oocytes (egg cells) of the Northern Leopard frog (Rana pipiens).

Ribonucleases are Author: Stefan Anitei. Journal Article: X-Radiation of Eggs of Rana Pipiens at Various Maturation Stages.

X-Radiation of Eggs of Rana Pipiens at Various Maturation Stages. Full Record; Other Related Research; Authors: Rollason, G.S. Publication Date: Sat Oct 01 EST Research Org. The U.S. Department of Energy's Office of Scientific and Technical Information.

BACKGROUND. The oocyte is the cellular precursor for the development of specialized cells. Oocytes from the South African clawed frog Xenopus laevis provide the most commonly used oocyte model for studying the properties, organization, and cellular roles of Ca 2+-permeable channels and experimentalists, the Xenopus oocyte brings together in a single cell the capacity to.

Rana pipiens Schreber, Northern Leopard Frog. James C. Rorabaugh 1. Historical versus Current Distribution. Northern leopard frogs (Rana pipiens) occurred historically from Newfoundland and southern Québec, south through New England to West Virginia, and west across the Canadian provinces and northern and central portions of the United States to British Columbia, Oregon, Washington.

We exposed R. pipiens larvae to different concentrations of atrazine (0, or 25 parts per billion, p.p.b.) in the laboratory by immersion (30 larvae per treatment; n = 3) from just after Cited by: Scientists in the field have conducted their research primarily by cloning metazoan nuclei into enucleated oocytes, a procedure developed originally in the leopard frog (Rana pipiens) by Robert Briggs and Thomas J.

King at the Institute for Cancer Research and the Lankenau Hospital Research Institute (ICR and LHRI), which is now the Fox Chase.

Abstract. For more than 30 yr, Xenopus laevis has been the animal of choice for studying the biochemical regulation of the meiotic and early mitotic vertebrate cell cycles. Attracted by its diploid genome, several laboratories have begun using the similar, although evolutionarily distinct, frog Xenopus tropicalis for studies of vertebrate development.

Comparisons between the two species Cited by: 5.Rana temporaria requires 20 days at - 5°C in order for “normal reproduction” to oc- cur. Prolonged cold exposure does not ap- pear to be an absolute requirement for ovulation and oocyte maturation in the northern leopard frog, R.

pipiens, and fer- tilizable eggs have long been obtained from prehibernatory fall Cited by: 9.Start studying Developmental Biology Gilbert 10th Edition Chapter 1. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Rana pipiens.

Frog template used in the book .